Biomarker Discovery:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
Recombinant:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
Purification:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
Blocking Assay:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
Staining:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
Cholesterol Assay:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
GSSG Assay:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
Enzyme-linked Immunosorbent Assay:Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit IgG (H + L) F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) Cell Signaling Technology Cat#4413S; RRID: AB_10694110 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) Cell Signaling Technology Cat#4409S; RRID: AB_1904022 Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) Cell Signaling Technology Cat#4408S; RRID: AB_10694704 Rat anti Mouse F4/80 antibody, clone A3-1 BioRad Cat#MCA497; RRID:AB_3065037 Rat anti Mouse CD206 antibody, clone MR5D3 BioRad Cat#MR5D3; RRID:AB_324449 Hamster anti Mouse CD11c antibody, clone N418 BioRad CatMCA1369; RRID:AB_1057759 BV421 Rat Anti-Mouse F4/80(T45-2342) BD Pharmingen Cat#565411; RRID:AB_2734779 BB515 Rat Anti-CD11b(M1/70) BD Pharmingen Cat#564454; RRID:AB_2665392 APC-Cy TM 7 Rat Anti-Mouse CD45 BD Pharmingen Cat#557659; RRID:AB_396774 Anti-UCP1 antibody Abcam Cat#AB234430; RRID:AB_2905638 Mouse Ly-6G/Ly-6C (Gr-1) Antibody R&D Systems Cat#MAB-1037; RRID:AB_2232806 4-Hydroxynonenal Antibody R&D Systems Cat#MAB3249-SP; RRID:AB_664165 Biological samples Human serum samples from Discovery Cohort, see Table S1 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 1, see Table S6 Shanghai Ruijin Hospital N/A Human serum samples from Validation Cohort 2, see Table S6 Shanghai Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Hematoxylin-eosin Servicebio Cat#G1005 Oil Red O Servicebio Cat#G1015 Sirius Red Servicebio Cat#GC307014 RNAiso Plus Takara Cat#9109 DAPI Beyotime Cat#C1002, CAS NO.:28718-90-3 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences Cat#553142; RRID:AB_394656 Fixable Viability Stain 510 BD Biosciences Cat#564406; RRID:AB_2869572 Sebacic acid Sigma-Aldrich Cat#283258 Guanidinoacetic acid Sigma-Aldrich Cat#G11608 Critical commercial assays Triglyceride assay kit Nanjing Jiancheng Bioengineering Institute Cat#A110-2-1 Total cholesterol assay kit Nanjing Jiancheng Bioengineering Institute Cat#A111-2-1 Alanine aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C009-3-1 Aspartate aminotransferase Assay Kit Nanjing Jiancheng Bioengineering Institute Cat#C010-3-1 DAB Substrate Kit Servicebio Cat#G1212-200T GSH and GSSG Assay Kit Beyotime Cat#S0053 M30-Apoptosense® ELISA kit PEVIVA Cat#10011 (Continued on next page) e1 Cell Reports Medicine 7, 102522, January 20, 2026 OPEN ACCESS ..
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